4HHT
T. maritima RNase H2 G21S in complex with nucleic acid substrate and calcium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.952, 47.626, 77.451 |
| Unit cell angles | 90.00, 131.26, 90.00 |
Refinement procedure
| Resolution | 26.015 - 3.100 |
| R-factor | 0.1818 |
| Rwork | 0.178 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.109 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 5292 |
| Completeness [%] | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 50 mM CaCl2, 0.1 M MES pH 6.0, 45% PEG200, VAPOR DIFFUSION, temperature 291K |
