4HHR
Crystal Structure of fatty acid alpha-dioxygenase (Arabidopsis thaliana)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 101.935, 101.935, 137.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.510 |
| R-factor | 0.15403 |
| Rwork | 0.153 |
| R-free | 0.18227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.067 | 0.527 |
| Number of reflections | 107022 | |
| <I/σ(I)> | 28.4 | 3 |
| Completeness [%] | 94.2 | 93.5 |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | 28% PEG400, 50mM Tris, 0.2M calcium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
