4HHL
High resolution crystal structure of Glucose Isomerase from Streptomyces sp. SK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.800, 91.680, 84.610 |
| Unit cell angles | 90.00, 119.65, 90.00 |
Refinement procedure
| Resolution | 48.140 - 1.730 |
| R-factor | 0.13378 |
| Rwork | 0.131 |
| R-free | 0.18786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1muw |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.795 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 1.900 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 74395 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 96.4 | 93.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | PEG 3350, Mg acetate, pH 7, VAPOR DIFFUSION, temperature 298.0K |
