4HH9
Anti-Human Cytomegalovirus (HCMV) Fab KE5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-09-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.860, 79.470, 86.270 |
| Unit cell angles | 90.00, 113.25, 90.00 |
Refinement procedure
| Resolution | 43.000 - 1.700 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.512 |
| Number of reflections | 97584 | |
| <I/σ(I)> | 19.04 | 3.8 |
| Completeness [%] | 96.8 | 95.5 |
| Redundancy | 7.33 | 7.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.05M Bis-Tris, pH 5.5, 20% PEG 3350, 0.2M Ammonium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
