4HH3
Structure of the AppA-PpsR2 core complex from Rb. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99981 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.780, 188.300, 51.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.907 - 1.750 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.005 | 0.684 |
| Number of reflections | 89594 | |
| <I/σ(I)> | 20.3 | 2.85 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 64 mM tri-sodium citrate, 10% PEG 5000 monomethyl ether, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
