4HGK
Shark IgNAR variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 127.980, 127.980, 151.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.055 - 3.040 |
| R-factor | 0.2387 |
| Rwork | 0.237 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ha2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.055 |
| High resolution limit [Å] | 3.040 |
| Rmerge | 0.220 |
| Number of reflections | 25935 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 16% PEG 2000 MME, 100 mM sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
