4HFW
Anti Rotavirus Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.100, 97.920, 94.230 |
| Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
| Resolution | 44.580 - 2.601 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23280 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.137 | |
| Number of reflections | 35536 | |
| <I/σ(I)> | 8.5 | 2.2 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 2.0 M Ammonium sulfate, 5% v/v 2-Propanol., pH 7.5, VAPOR DIFFUSION, temperature 291K |
