4HFI

The GLIC pentameric Ligand-Gated Ion Channel at 2.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	CCD
Collection date	2010-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9193
Spacegroup name	C 1 2 1
Unit cell lengths	181.890, 133.200, 160.200
Unit cell angles	90.00, 102.74, 90.00

Refinement procedure

Resolution	54.050 - 2.400
R-factor	0.2054
Rwork	0.205
R-free	0.21610
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.700	70.700	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge	0.084	0.037	0.672
<I/σ(I)>	11.5	47.4	1.7
Completeness [%]	98.9	94.9	95.3
Redundancy	4.8	5.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	12-15% PEG4K, 0.1M Na Acetate pH4, 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K