4HFE
The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to ethanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9194 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.990, 133.620, 159.130 |
| Unit cell angles | 90.00, 102.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.2055 |
| Rwork | 0.205 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.600 |
| Number of reflections | 90680 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 0.2M Ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
