4HEM
Llama vHH-02 binder of ORF49 (RBP) from lactococcal phage TP901-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.980, 104.250, 122.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.160 - 1.650 |
| R-factor | 0.1677 |
| Rwork | 0.167 |
| R-free | 0.17720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hg0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.054 | 0.530 |
| Number of reflections | 108049 | |
| <I/σ(I)> | 26 | 5.7 |
| Completeness [%] | 90.7 | 97.5 |
| Redundancy | 15 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 30% PEG 4000, 0.2M ammonium sulfate, 0.1M Na acetate, 4.7 mg/ml Protein complex , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
