Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790, 1.604 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.354, 46.354, 174.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.620 - 1.600 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.620 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 21120 | |
| <I/σ(I)> | 15.4 | 1.26 |
| Completeness [%] | 80.0 | 18.5 |
| Redundancy | 4.1 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 1.2M AMMONIUM SULFATE, 0.01 M CO(II) CL2, 0.1M MES 6.5 ADDITIVE 2M SODIUM THIOCYANATE MIXED ABOVE COMPONENTS WITH EQUAL VOLUME OF PROTEIN, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
