4HE8
Crystal structure of the membrane domain of respiratory complex I from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.260, 120.540, 176.660 |
| Unit cell angles | 91.91, 95.73, 101.41 |
Refinement procedure
| Resolution | 25.725 - 3.300 |
| R-factor | 0.2099 |
| Rwork | 0.209 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rko |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1041)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.077 | 0.737 |
| Number of reflections | 99359 | |
| <I/σ(I)> | 5.9 | 0.9 |
| Completeness [%] | 96.4 | 92.6 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 100 mM phosphate-citrate pH 4.5, 26% (v/v) PEG300, 5 mM CHAPS, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
