4HE7
Crystal Structure of Brazzein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1995-01-01 |
Detector | WEISSENBERG |
Wavelength(s) | 1.04 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 61.400, 61.400, 59.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.800 - 1.800 |
R-factor | 0.21933 |
Rwork | 0.218 |
R-free | 0.25162 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2brz |
RMSD bond length | 0.018 |
RMSD bond angle | 2.124 |
Data reduction software | HKL-2000 |
Data scaling software | WEIS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 1.847 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 6728 | |
Completeness [%] | 89.4 | 83.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.9-1.0M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |