4HDQ
Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and the Heart of Glass (HEG1) cytoplasmic tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.310, 77.410, 58.570 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 29.150 - 1.950 |
| R-factor | 0.20342 |
| Rwork | 0.200 |
| R-free | 0.26278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hdo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.932 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.066 | 0.347 |
| Number of reflections | 36963 | |
| <I/σ(I)> | 20.99 | 3.9 |
| Completeness [%] | 99.1 | 96.8 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 15% PEG2000 MME, 100 mM Tris, 100 mM potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
