4HDO
Crystal structure of the binary Complex of KRIT1 bound to the Rap1 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.850, 77.750, 58.850 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 32.430 - 1.670 |
| R-factor | 0.21253 |
| Rwork | 0.212 |
| R-free | 0.23142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c1y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.046 | 0.350 |
| Number of reflections | 57913 | |
| <I/σ(I)> | 31.27 | 4.37 |
| Completeness [%] | 96.0 | 80 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 15% PEG2000 MME, 100 mM Tris, 100 mM potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
