4HCZ

PHF1 Tudor in complex with H3K36me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-01-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541
Spacegroup name	C 1 2 1
Unit cell lengths	88.030, 29.670, 62.000
Unit cell angles	90.00, 116.41, 90.00

Refinement procedure

Resolution	23.413 - 1.850
R-factor	0.2058
Rwork	0.204
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e5p
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.413	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	12091
Completeness [%]	96.4	81.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	0.1 M HEPES pH 7.5, 20% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K