4HCX

Structure of ICDH-1 from M.tuberculosis complexed with NADPH & Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2012-07-21
Detector	FUJI
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	90.135, 92.704, 102.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.180
Rwork	0.205
R-free	0.26171
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.180
Number of reflections	42831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	30% PEG2K, 0.1M Tris HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K