4HCN
Crystal structure of Burkholderia pseudomallei effector protein CHBP in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.431, 45.185, 67.886 |
| Unit cell angles | 90.00, 118.46, 90.00 |
Refinement procedure
| Resolution | 19.350 - 2.600 |
| R-factor | 0.222 |
| Rwork | 0.218 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1UBQ AND PDB ENTRY: 3EIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.134 |
| Data reduction software | DENZO |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 10218 | |
| Completeness [%] | 95.3 | 93.1 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.2 | 293 | 12.5% PEG3350, 125MM MAGNESIUM FORMATE, pH 7.2, EVAPORATION, temperature 293K |
