4HCI
Uncharacterized Cupredoxin-like Domain Protein Cupredoxin_1 from Bacillus anthracis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97926 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.200, 42.809, 51.245 |
Unit cell angles | 90.00, 120.40, 90.00 |
Refinement procedure
Resolution | 24.521 - 1.630 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20200 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 21617 | |
<I/σ(I)> | 19.9 | 0.508 |
Completeness [%] | 96.8 | 93.2 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 2.86 M ammonium nitrate, 150 mM Tris pH 8.0, 100 mM sodium iodide, VAPOR DIFFUSION, SITTING DROP, temperature 289K |