4HBT
Crystal structure of native CTX-M-15 extended-spectrum beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.698, 45.720, 117.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.270 - 1.100 |
| R-factor | 0.11789 |
| Rwork | 0.117 |
| R-free | 0.13644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iys |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.935 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.273 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 97955 | |
| Completeness [%] | 99.8 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCl, 2.2-2.4M ammonium sulfate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
