4HAH

Structure of the Salmonella plasmid virulence C protein (SpvC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2011-05-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.528, 71.912, 106.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.264 - 1.800
R-factor	0.1952
Rwork	0.194
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.264	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.107	0.485
Number of reflections	45887
<I/σ(I)>	9	2.5
Completeness [%]	98.4	86.8
Redundancy	7.22	4.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	20% PEG 3350 (a 50% w/v solution) 0.1M Ammonium Chloride (1M stock solution) , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K