4HAA
Structure of Ribonuclease Binase Glu43Ala/Phe81Ala Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 43 |
| Unit cell lengths | 50.360, 50.360, 196.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.730 - 1.900 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gov |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.782 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.010 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.091 | 0.492 |
| Number of reflections | 37967 | |
| <I/σ(I)> | 2.49 | |
| Completeness [%] | 97.7 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 10 mg/mL protein, reservoir: 0.1 M citric acid, pH 3.5, 3 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
