4H9M
The first Jack bean urease (Canavalia ensiformis) complex obtained at 1.52 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2012-08-09 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 140.391, 140.391, 198.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.610 - 1.520 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gy7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.075 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.518 | 1.520 |
| Rmerge | 0.093 | |
| Number of reflections | 223658 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 293 | 1.6M ammonium phosphate dibasic, 100mM Tris pH 8.8, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
