4H9K

Crystal structure of cleavage site mutant of Npro of classical swine fever virus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2011-12-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.489, 58.298, 75.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.700 - 1.599
R-factor	0.1824
Rwork	0.179
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h9j
RMSD bond length	0.006
RMSD bond angle	1.055
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.700	1.630
High resolution limit [Å]	1.590	1.600
Number of reflections	24710
<I/σ(I)>	40.6	1.96
Completeness [%]	99.2	87.9
Redundancy	5.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	25% PEG3350, 0.2 M (NH4)2SO4 and 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K