4H9J

Crystal structure of N-terminal protease (Npro) of classical swine fever virus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-01
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	60.133, 65.398, 33.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.685 - 1.600
R-factor	0.1744
Rwork	0.171
R-free	0.20830
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.123
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	1.620
High resolution limit [Å]	1.580	1.580
Number of reflections	18840
<I/σ(I)>	12.63	1.98
Completeness [%]	99.9	99.86
Redundancy	34.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	25% PEG3350, 0.2 M (NH4)2SO4 and 0.1 M Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K