4H9J
Crystal structure of N-terminal protease (Npro) of classical swine fever virus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.133, 65.398, 33.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.685 - 1.600 |
| R-factor | 0.1744 |
| Rwork | 0.171 |
| R-free | 0.20830 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.760 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 18840 | |
| <I/σ(I)> | 12.63 | 1.98 |
| Completeness [%] | 99.9 | 99.86 |
| Redundancy | 34.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 290 | 25% PEG3350, 0.2 M (NH4)2SO4 and 0.1 M Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
