4H8M

Crystal structure of a parallel 6-helix coiled coil CC-Hex-H24-A5/7C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Detector technology	CCD
Collection date	2011-07-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	H 3
Unit cell lengths	31.700, 31.700, 132.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.013 - 1.429
R-factor	0.1973
Rwork	0.197
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r46
RMSD bond length	0.002
RMSD bond angle	0.573
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.013
High resolution limit [Å]	1.429
Number of reflections	9078

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	30 mM sodium fluoride, 30 mM sodium bromide, 30 mM sodium iodide, 50 mM sodium HEPES, 50 mM MOPS (acid) pH 7.5, 20 % PEG-MME 550 and 10 % PEG 20K, VAPOR DIFFUSION, SITTING DROP, temperature 291K