4H7Y

Crystal structure of the tetratricopeptide repeat (TPR) motif of human dual specificity protein kinase Mps1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	79.502, 80.250, 141.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.500 - 1.800
R-factor	0.1992
Rwork	0.199
R-free	0.22570
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.500	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.036	0.572
Number of reflections	80100
<I/σ(I)>	20.1
Completeness [%]	98.8	98.6
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	20% PEG 3350, 100 mM MES, 250 mM KCl, pH 6.0, VAPOR DIFFUSION, temperature 291K