4H7X
Crystal structure of the tetratricopeptide repeat (TPR) motif of human dual specificity protein kinase Mps1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9497 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.487, 79.487, 137.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.700 - 2.600 |
| R-factor | 0.1975 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.063 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.7.3_928) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.156 | 0.599 |
| Number of reflections | 14021 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 20% PEG 3350, 100 mM MES, 250 mM KCl, pH 6.0, VAPOR DIFFUSION, temperature 291K |
