4H7K
Crystal structure of haloalkane dehalogenase LinB I253M mutant from Sphingobium sp. MI1205
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Collection date | 2010-03-02 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 50.538, 72.153, 73.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.17371 |
Rwork | 0.172 |
R-free | 0.20463 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h77 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.298 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 27832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | VAPOR DIFFUSION, SITTING DROP, temperature 278K |