4H6Y

Crystal structure of the DH-PH-PH domain of FARP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 32
Unit cell lengths	142.237, 142.237, 105.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.574 - 4.090
R-factor	0.2902
Rwork	0.289
R-free	0.31730
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.718
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.170
High resolution limit [Å]	4.090	11.090	4.090
Rmerge	0.107	0.069	0.506
Number of reflections	18762
<I/σ(I)>	10
Completeness [%]	99.5	97.4	97.7
Redundancy	7.7	7.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	100 mM MES, 0.2 M Mg formate, 14% PEG3350, pH 6.4, vapor diffusion, hanging drop, temperature 293K