4H62
Structure of the Saccharomyces cerevisiae Mediator subcomplex Med17C/Med11C/Med22C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97972 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 261.230, 261.230, 47.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.308 - 3.000 |
| R-factor | 0.2056 |
| Rwork | 0.204 |
| R-free | 0.22850 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.615 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.061 | 0.692 |
| Number of reflections | 24338 | |
| <I/σ(I)> | 16.46 | 0.56 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 4 M ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
