4H61

Structure of the Schizosaccharomyces pombe Mediator subunit Med6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.9189
Spacegroup name	P 21 3
Unit cell lengths	125.950, 125.950, 125.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.060 - 2.700
R-factor	0.183
Rwork	0.181
R-free	0.21720
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.060	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.052	1.010
Number of reflections	18565
<I/σ(I)>	41.4	0.6
Completeness [%]	100.0	100
Redundancy	20	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES, 400 mM sodium citrate , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K