4H5Y

High-resolution crystal structure of Legionella pneumophila LidA (60-594)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	P 21 21 21
Unit cell lengths	55.554, 64.468, 164.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.026 - 2.100
R-factor	0.2033
Rwork	0.201
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.773
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.187	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	35304
Completeness [%]	99.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	200 mM trimethylamine N-oxide, pH 8.0, 20% w/v PEG2000 MME, 1% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K