4H5L
Crystal Structure of Toscana Virus Nucleocapsid Protein Hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.350, 93.750, 95.520 |
| Unit cell angles | 67.92, 85.94, 87.95 |
Refinement procedure
| Resolution | 50.220 - 2.750 |
| R-factor | 0.2199 |
| Rwork | 0.219 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lyf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 88.345 | 50.221 | 2.900 |
| High resolution limit [Å] | 2.750 | 8.700 | 2.750 |
| Rmerge | 0.026 | 0.474 | |
| Total number of observations | 2453 | 10787 | |
| Number of reflections | 39945 | ||
| <I/σ(I)> | 6.8 | 22.6 | 1.4 |
| Completeness [%] | 95.2 | 95.9 | 94.2 |
| Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23% isopropanol, 280 mM magnesium chloride, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
