4H5B
Crystal Structure of DR_1245 from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91983 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.714, 91.714, 87.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.900 - 2.000 |
| R-factor | 0.16524 |
| Rwork | 0.164 |
| R-free | 0.19480 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 28448 | |
| Completeness [%] | 99.8 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 1.7 M NH4(SO4)2, 27% w/v glycerol, 100 mM KBr, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
