Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.25363 |
| Spacegroup name | P 3 |
| Unit cell lengths | 129.530, 129.530, 76.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.280 - 2.500 |
| R-factor | 0.22331 |
| Rwork | 0.219 |
| R-free | 0.29778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vea |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.119 | 0.515 |
| Number of reflections | 57983 | |
| <I/σ(I)> | 15.7 | 1.1 |
| Completeness [%] | 90.0 | 52 |
| Redundancy | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 30% PEG 2K MME, 0.1M HEPES PH7.6, 0.5M KBR, 10MM ZINC ACETATE, 0.2M L-HIS, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
