4H4B
Human cytosolic 5'-nucleotidase II in complex with Anthraquinone-2,6- disulfonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979696 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.110, 126.830, 130.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.900 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j2c |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.659 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.900 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 16022 |
| Completeness [%] | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | magnesium sulphate and Tris buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
