4H3P
Crystal structure of human ERK2 complexed with a MAPK docking peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-31 |
| Detector | PSI PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.480, 58.770, 79.180 |
| Unit cell angles | 100.93, 98.96, 90.01 |
Refinement procedure
| Resolution | 42.370 - 2.300 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tei |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.370 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 30568 | |
| Completeness [%] | 94.9 | 93.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 25-30% PEG6000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
