4H3N
mPlumAYC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2012-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5418, 1.5621, 1.7321 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.720, 61.550, 96.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.920 - 1.750 |
| R-factor | 0.22434 |
| Rwork | 0.221 |
| R-free | 0.28104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qlg |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.999 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.920 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 22710 |
| Completeness [%] | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.3 uL + 0.3 uL drops, precipitant: 200 mM sodium cacodylate, 100 mM Tris-HCl, pH 8.5, 30% (w/v) PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
