4H2Z
Crystal structure of human GLTP bound with 12:0 monosulfatide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.605, 49.998, 65.457 |
| Unit cell angles | 90.00, 118.62, 90.00 |
Refinement procedure
| Resolution | 14.970 - 1.450 |
| R-factor | 0.16212 |
| Rwork | 0.160 |
| R-free | 0.19971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rzn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 38717 | |
| Completeness [%] | 97.8 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15-20% pEG 3350, 0.1M MES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
