4H2K
Crystal structure of the catalytic domain of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.667, 44.721, 92.481 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.840 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3isz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.035 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.090 | |
| Number of reflections | 42453 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.5 | 97.8 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 289 | 0.2 M Ammonium acetate, 25% PEG 3350, 0.1 M BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
