4H1U

Nucleotide-free human dynamin-1-like protein GTPase-GED fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-02
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 2
Unit cell lengths	53.500, 151.430, 42.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.853 - 2.300
R-factor	0.2317
Rwork	0.230
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3snh
RMSD bond length	0.005
RMSD bond angle	0.976
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.715	36.715	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.044	0.449
Total number of observations		1728	7783
Number of reflections	15371
<I/σ(I)>	7.9	11.8	1.7
Completeness [%]	96.6	90.6	96.8
Redundancy	3.5	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M sodium citrate, 27.5% PEG 3000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K