4H0P
Crystal Structure of Acetate Kinase from Cryptococcus neoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12712 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.370, 107.613, 79.108 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 38.973 - 1.894 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g99 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.950 | 1.970 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 64297 | |
| <I/σ(I)> | 27 | 5.2 |
| Completeness [%] | 95.9 | 89.8 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 18.5% PEG2000, 100 mM sodium tartrate, 50 mM ADA, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
