4H09

Crystal structure of a leucine-rich repeat protein (EUBVEN_01088) from Eubacterium ventriosum ATCC 27560 at 2.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97925
Spacegroup name	P 61
Unit cell lengths	105.513, 105.513, 361.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.965 - 2.500
R-factor	0.1775
Rwork	0.176
R-free	0.20930
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.965	29.965	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge		0.049	0.630
Total number of observations		3278	22837
Number of reflections	78283
<I/σ(I)>	9.4	11.1	1.2
Completeness [%]	99.9	93	100
Redundancy	4	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1M sodium citrate pH 5.6, 20% 2-propanol, 20% polyethylene glycol 4000, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K