4H05
Crystal structure of aminoglycoside-3'-phosphotransferase of type VIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.340, 103.340, 53.680 |
| Unit cell angles | 90.00, 94.36, 90.00 |
Refinement procedure
| Resolution | 28.970 - 2.150 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3R78 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.348 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.970 | 2.206 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 30335 | |
| <I/σ(I)> | 2.63 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 278 | 0.1M Sodium citrate; 18% PEG20K, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
