4GZZ
Crystal structures of bacterial RNA Polymerase paused elongation complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91956 |
| Spacegroup name | H 3 |
| Unit cell lengths | 286.549, 286.549, 199.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.785 - 4.293 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.934 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 10.810 | 4.000 |
| Rmerge | 0.442 | 0.719 | |
| Number of reflections | 51877 | ||
| <I/σ(I)> | 4.9 | ||
| Completeness [%] | 99.5 | 99.8 | 97.7 |
| Redundancy | 5.9 | 5.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 5-7% PEG 3000, 0.1M MES pH 6.1-6.3, 0.3M LiCl, 10mM MgCl2, vapor diffusion, hanging drop, temperature 295K |
