4GZI
Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-02 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.81620 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.895, 49.273, 57.075 |
| Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.680 |
| R-factor | 0.1375 |
| Rwork | 0.136 |
| R-free | 0.17130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ur7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.567 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.069 | 0.264 |
| Number of reflections | 34231 | |
| <I/σ(I)> | 23.2 | 5.1 |
| Completeness [%] | 99.0 | 92.7 |
| Redundancy | 6.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1 M sodium citrate, 0.2 M ammonium acetate, 24% PEG 8000, 0.2 mM laminarahexose, streak seeding, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
