4GZE
Crystal structure of 6-phospho-beta-glucosidase from Lactobacillus plantarum (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 |
| Unit cell lengths | 96.086, 96.086, 289.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.310 |
| R-factor | 0.17421 |
| Rwork | 0.174 |
| R-free | 0.21338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qom |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.079 | 0.285 |
| Number of reflections | 130353 | |
| <I/σ(I)> | 21.8 | 2.75 |
| Completeness [%] | 99.6 | 96.7 |
| Redundancy | 4.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.6 M NaCl, 0.1 M MES/NaOH, 20% PEG4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
