4GZ1

Mus Musculus Tdp2 reaction product (5'-phosphorylated DNA)-Mg2+ complex at 1.5 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-03
Detector	MAR300
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	55.200, 69.139, 167.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.073 - 1.500
R-factor	0.1468
Rwork	0.145
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	104060
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	12% PEG 3350, 100mM HEPES, 50mM Mg(CO2)2, pH 7, vapor diffusion, temperature 293K