4GYX
The von Willebrand Factor A3 domain binding region of type III collagen stabilized by the cysteine knot
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-29 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 19.070, 26.990, 64.120 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 19.050 - 1.490 |
| R-factor | 0.1805 |
| Rwork | 0.176 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dmt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.146 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.050 | 19.050 | 1.570 |
| High resolution limit [Å] | 1.490 | 4.710 | 1.490 |
| Rmerge | 0.083 | 0.068 | 0.383 |
| Number of reflections | 10752 | ||
| <I/σ(I)> | 8.2 | 14.5 | 3.2 |
| Completeness [%] | 98.9 | 98.4 | 93.1 |
| Redundancy | 3.5 | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 28% PEG 3350, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
